Cool Image: Lighting up Brain Cells

Neurons activated with red or blue light.

Neurons activated with red or blue light using algae-derived opsins. Credit: Yasunobu Murata/McGovern Institute for Brain Research at MIT.

The nerve cells, or neurons, lit up in blue and red in this image of mouse brain tissue are expressing algae-derived, light-sensitive proteins called opsins. To control neurons with light, scientists engineer the cells to produce particular opsins, most of which respond to light in the blue-green range. Then they shine light on the cell to activate it. Now, a team of researchers led by Ed Boyden of the Massachusetts Institute of Technology and Gane Ka-Shu Wong of the University of Alberta has discovered an opsin that responds to red light preferentially, enabling them to manipulate two groups of neurons simultaneously with different colors of light and get a more comprehensive look at how those two sets of brain cells interact. Other opsins have shown potential for vision restoration in animal studies, and, because red light causes less damage to tissue than blue-green light, this new opsin might eventually be used for such treatments in humans.

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Cool Image: Denying Microbial Moochers

V. cholerae and V. cholerae

Productive V. cholerae (yellow) and exploitive V. cholerae (red). Credit: Carey Nadell, Princeton University.

What looks like an abstract oil painting is actually an image of several cholera-causing V. cholerae bacterial communities. These communities, called biofilms, include productive and exploitive microbial members. The industrious bacteria (yellow) tend to thrive in denser biofilms (top) while moochers (red) thrive in weaker biofilms (bottom). In an effort to understand this phenomenon, Princeton University researchers led by Bonnie Bassler Exit icon discovered two ways the freeloaders are denied food. They found that some V. cholerae cover themselves with a thick coating to prevent nutritious carbon- and nitrogen-containing molecules from drifting over to the scroungers. In addition, the natural flow of fluids over biofilms can wash away any leftovers. Encouraging such bacterial fairness could boost the efficient breakdown of organic materials into useful products, such as biofuels. On the other hand, counteracting it could lead to better treatment of illnesses, like cholera, by starving the most productive bacteria and thereby weakening the infection.

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Cool Image: Visualizing Viral Activity

Viral RNA (red) in an RSV-infected cell. Credit: Eric Alonas and Philip Santangelo, Georgia Institute of Technology and Emory University.

Viral RNA (red) in an RSV-infected cell. Credit: Eric Alonas and Philip Santangelo, Georgia Institute of Technology and Emory University.

What looks like a colorful pattern produced as light enters a kaleidoscope is an image of a cell infected with respiratory syncytial virus (RSV) illuminated by a new imaging technology. Although relatively harmless in most children, RSV can lead to bronchitis and pneumonia in others. Philip Santangelo Exit icon of the Georgia Institute of Technology and Emory University, along with colleagues nationwide, used multiply-labeled tetravalent RNA imaging probes (MTRIPS) to observe the entry, assembly and replication of RSV inside a living cell. Once introduced into RSV-plagued cells, the MTRIPS latched onto the viral RNA (in the image, red) without altering the level of infectivity. This led to fluorescent RSV viral particles that let the researchers track the viral RNA in host cells and better understand what the virus was doing. The knowledge gained from this new technique might aid in the development of RSV antiviral drugs and possibly a vaccine. Scientists could also one day use the imaging approach to study other RNA viruses, such as the flu and Ebola.

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Cool Images: Holiday Season Cells

Yeast cells deficient in zinc and the Tsa1 protein have protein tangles. Credit: Colin MacDiarmid and David Eide, University of Wisconsin-Madison.

Yeast cells deficient in zinc and the Tsa1 protein have protein tangles. Credit: Colin MacDiarmid and David Eide, University of Wisconsin-Madison.

Just in time for the holidays, we’ve wrapped up a few red and green cellular images from basic research studies. In this snapshot, we see a group of yeast cells that are deficient in zinc, a metal that plays a key role in creating and maintaining protein shape. The cells also lack a protein called Tsa1, which normally keeps proteins from sticking together. Green areas highlight protein tangles caused by the double deficiency. Red outlines the cells. Protein clumping plays a role in many human diseases, including Parkinson’s and Alzheimer’s, so knowledge of why it happens—and what prevents it in healthy cells—could aid the development of treatments.

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Cool Image: Tick Tock, Master Clock

Master clock in mouse brain with the nuclei of the clock cells shown in blue and the VIP molecule shown in green. Credit: Cristina Mazuski in the lab of Erik Herzog, Washington University in St. Louis.

Master clock in mouse brain with the nuclei of the clock cells shown in blue and the VIP molecule shown in green. Credit: Cristina Mazuski in the lab of Erik Herzog, Washington University in St. Louis.

Our biological clocks play a large part in influencing our sleep patterns, hormone levels, body temperature and appetite. A small molecule called VIP, shown in green, enables time-keeping neurons in the brain’s central clock to coordinate daily rhythms. New research shows that, at least in mice, higher doses of the molecule can cause neurons to get out of synch. By desynchronizing mouse neurons with an extra burst of VIP, Erik Herzog Exit icon of Washington University in St. Louis found that the cells could better adapt to abrupt changes in light (day)-dark (night) cycles. The finding could one day lead to a method to reduce jet lag recovery times and help shift workers better adjust to schedule changes.

Learn more:

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Circadian Rhythms Fact Sheet
Tick Tock: New Clues About Biological Clocks and Health Article from Inside Life Science
A Light on Life’s Rhythms Article from Findings Magazine

Healing Wounds, Growing Hair

Wound healing in process. Credit: Yaron Fuchs and Samara Brown in the lab of Hermann Steller, Rockefeller University.

Credit: Yaron Fuchs and Samara Brown in the lab of Hermann Steller, Rockefeller University.

Whether injured by a scrape, minor burn or knife wound, skin goes through the same steps to heal itself. Regrowing hair over new skin is one of the final steps. All the hair you can see on your body is non-living, made up of “dead” cells and protein. It sprouts from living cells in the skin called hair follicle stem cells, shown here in red and orange. For more pictures of hair follicle stem cells—and many other stunning scientific images and videos—go to the NIGMS Image and Video Gallery.

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Cool Video: How Bee Venom Toxin Kills Cells

Credit: Huey Huang, Rice University.

Credit: Huey Huang, Rice University.

A new video, starring the toxin in bee venom, might help scientists design new drugs to combat bacterial infections. The video, by Rice University biophysicist Huey Huang Exit icon, condenses 6.5 minutes into less than a minute to show how the toxin, called melittin, destroys an animal or bacterial cell.

What looks like a red balloon is an artificial cell filled with red dye. Melittin molecules are colored green and float on the cell’s surface like twigs on a pond. As melittin accumulates on the cell’s membrane, the membrane expands to accommodate it. In the video, the membrane stretches into a column on the left.

When melittin levels reach a critical threshold, countless pinhole leaks burst open in the membrane. The cell’s vital fluids—red dye in the video—leak out through these pores. Within minutes, the cell collapses.

Many organisms use such a pore-forming technique to kill attacking bacterial cells. This research reveals molecular-level details of the strategy, bringing pharmaceutical scientists a step closer to harnessing it in the design of new antibiotics.

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Cool Image: Tiny Bacterial Motor

Phillip Klebba, Kansas State University.

Credit: Phillip Klebba, Kansas State University.

It looks like a fluorescent pill, but this image of an E. coli cell actually shows a new potential target in the fight against infectious diseases. The green highlights a protein called TonB, which is produced by many gram-negative bacteria, including those that cause typhoid fever, meningitis and dysentery. TonB lets bacteria take up iron from the host’s body, which they need to survive. New research from Phillip Klebba of Kansas State University and his colleagues shows how TonB powers iron uptake. When TonB spins within the cell envelope (the bacteria’s “skin”) like a tiny motor, it produces energy that lets another protein pull iron into the cell. This knowledge may lead to the development of antibiotics that block the motion of TonB, potentially stopping an infection in its tracks.

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